Glial cell line-derived neurotrophic factor (GDNF) gene expression in the human brain: A post mortem in situ hybridization study with special reference to Parkinson's disease
Identifieur interne : 002175 ( Main/Exploration ); précédent : 002174; suivant : 002176Glial cell line-derived neurotrophic factor (GDNF) gene expression in the human brain: A post mortem in situ hybridization study with special reference to Parkinson's disease
Auteurs : S. Hunot [France] ; V. Bernard [France] ; B. Faucheux [France] ; F. Boissière [France] ; E. Leguern [France] ; C. Brana [France] ; P. Gautris [France] ; J. Guérin [France] ; B. Bloch [France] ; Yves Agid [France] ; C. Hirsch [France]Source :
- Journal of Neural Transmission [ 0300-9564 ] ; 1996-08-01.
Abstract
Summary: Glial cell line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor for dopaminergic neurons. Since dopaminergic neurons degenerate in Parkinson's disease, this factor is a potential therapeutical tool that may save dopaminergic neurons during the pathological process. Moreover, a reduced GDNF expression may be involved in the pathophysiology of the disease. In this study, we tested whether altered GDNF production may participate in the mechanism of cell death in this disease. GDNF gene expression was analyzed by in situ hybridization using riboprobes corresponding to a sequence of the exon 2 human GDNF gene. Experiments were performed on tissue sections of the mesencephalon and the striatum from 8 patients with Parkinson's disease and 6 control subjects matched for age at death and for post mortem delay. No labelling was observed in either group of patients. This absence of detectable expression could not be attributed to methodological problems as a positive staining was observed using the same probes for sections of astroglioma biopsies from human adults and for sections of a newborn infant brain obtained at post-mortem. These data suggest that GDNF is probably expressed at a very low level in the adult human brain and its involvement in the pathophysiology of Parkinson's disease remains to be demonstrated. GDNF may represent a powerful new therapeutic agent for Parkinson's disease, however.
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DOI: 10.1007/BF01291789
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Hirsch</name><affiliation wicri:level="3"><country>France</country><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName><wicri:orgArea>INSERM U289, Hôpital de la Salpêtrière</wicri:orgArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Neural Transmission</title><title level="j" type="sub">Basic neurosciences and genetics, Parkinson's disease and allied conditions, Alzheimer's disease and related disorders, biological psychiatry</title><title level="j" type="abbrev">J. Neural Transmission</title><idno type="ISSN">0300-9564</idno><idno type="eISSN">1435-1463</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1996-08-01">1996-08-01</date><biblScope unit="volume">103</biblScope><biblScope unit="issue">8-9</biblScope><biblScope unit="page" from="1043">1043</biblScope><biblScope unit="page" to="1052">1052</biblScope></imprint><idno type="ISSN">0300-9564</idno></series><idno type="istex">B0946E45290010B99F49360D74F35951B07973C5</idno><idno type="DOI">10.1007/BF01291789</idno><idno type="ArticleID">Art10</idno><idno type="ArticleID">BF01291789</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0300-9564</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Glial cell line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor for dopaminergic neurons. Since dopaminergic neurons degenerate in Parkinson's disease, this factor is a potential therapeutical tool that may save dopaminergic neurons during the pathological process. Moreover, a reduced GDNF expression may be involved in the pathophysiology of the disease. In this study, we tested whether altered GDNF production may participate in the mechanism of cell death in this disease. GDNF gene expression was analyzed by in situ hybridization using riboprobes corresponding to a sequence of the exon 2 human GDNF gene. Experiments were performed on tissue sections of the mesencephalon and the striatum from 8 patients with Parkinson's disease and 6 control subjects matched for age at death and for post mortem delay. No labelling was observed in either group of patients. This absence of detectable expression could not be attributed to methodological problems as a positive staining was observed using the same probes for sections of astroglioma biopsies from human adults and for sections of a newborn infant brain obtained at post-mortem. These data suggest that GDNF is probably expressed at a very low level in the adult human brain and its involvement in the pathophysiology of Parkinson's disease remains to be demonstrated. GDNF may represent a powerful new therapeutic agent for Parkinson's disease, however.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Bordeaux</li><li>Paris</li></settlement><orgName><li>Hôpital de la Salpêtrière</li></orgName></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Hunot, S" sort="Hunot, S" uniqKey="Hunot S" first="S." last="Hunot">S. Hunot</name></region><name sortKey="Agid, Y" sort="Agid, Y" uniqKey="Agid Y" first="Y." last="Agid">Yves Agid</name><name sortKey="Bernard, V" sort="Bernard, V" uniqKey="Bernard V" first="V." last="Bernard">V. Bernard</name><name sortKey="Bernard, V" sort="Bernard, V" uniqKey="Bernard V" first="V." last="Bernard">V. Bernard</name><name sortKey="Bloch, B" sort="Bloch, B" uniqKey="Bloch B" first="B." last="Bloch">B. Bloch</name><name sortKey="Boissiere, F" sort="Boissiere, F" uniqKey="Boissiere F" first="F." last="Boissière">F. Boissière</name><name sortKey="Brana, C" sort="Brana, C" uniqKey="Brana C" first="C." last="Brana">C. Brana</name><name sortKey="Faucheux, B" sort="Faucheux, B" uniqKey="Faucheux B" first="B." last="Faucheux">B. Faucheux</name><name sortKey="Gautris, P" sort="Gautris, P" uniqKey="Gautris P" first="P." last="Gautris">P. Gautris</name><name sortKey="Guerin, J" sort="Guerin, J" uniqKey="Guerin J" first="J." last="Guérin">J. Guérin</name><name sortKey="Hirsch, C" sort="Hirsch, C" uniqKey="Hirsch C" first="C." last="Hirsch">C. Hirsch</name><name sortKey="Leguern, E" sort="Leguern, E" uniqKey="Leguern E" first="E." last="Leguern">E. Leguern</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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